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Understanding Flow Cytometry: A Powerful Tool in Biomedical Science
Flow cytometry is a sophisticated technology widely used in biomedical research and clinical diagnostics to analyze the physical and chemical characteristics of cells or particles. At its core, flow cytometry allows scientists to examine thousands of individual cells per second, providing insights into cell size, complexity, phenotype, and functional state. The ability to analyze cells rapidly and quantitatively has made flow cytometry indispensable in fields ranging from immunology to oncology.
The principle behind flow cytometry involves suspending cells in a fluid stream and passing them through a laser beam. As each cell passes through the beam, it scatters light and may emit fluorescence if it has been tagged with specific fluorescent markers. Detectors capture this scattered and emitted light, converting it into electronic signals that are analyzed to generate detailed information about each cell’s characteristics. This multiparametric analysis allows researchers to…